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KMID : 0545119990090060804
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 6 p.804 ~ p.810
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Induction by Carvone of the Polychlorinated Biphenyl(PCB)-Degradative Pathway in Alcaligenes eutrophus H850 and Its Molecular Monitoring
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PARK, YOUNG-IN
SO, JAE-SEONG/KOH, SUNG-CHEOL
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Abstract
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There is a possibility that carvone, a monoterpene from spearmint (Mentha spicata), could induce the bph degradative pathway and genes in Alcaligenes eutrophus H850, which is a known Gram-negative PCB degrader with a broad substrate specificity that was thoroughly investigated with Arthrobacter sp. B1B, a Gram-positive PCB degrader. The strains B1B and H850 were unable to utilize and grow on the plant terpene [(R)-(-)-carvone] (50ppm) to be recognized as a sole carbon source. Nevertheless, the carvone did induce 2,3-dihydroxybiphenyl 1,2-dioxygenase (encoded by bphC) in the strain B1B, as observed by a resting cell assay that monitors accumulation of a yellow meta ring fission product from 4,4´-dichiorobiphenyl (DCBp). The monoterpene, however, did not appear to induce the meta cleavage pathway in the strain H850. Instead, an assumption was made that the strain might be using an alternative pathway, probably the orthocleavage pathway. A reverse transcription (RT)-PCR system, utilizing primers designed from a conserved region of the bphC gene of Arthrobacter sp. M5, was employed to verify the occurrence of the alternative pathway. A successful amplification (182bp) of mRNA transcribed from the N-terminal region of the bphC gene was accomplished in H850 cells induced by carvone (50ppm) as well as in biphenylgrowth cells. It is, therefore, likely that H850 possesses a specific PCB degradation pathway and hence a different substrate specificity compared with BIB. This study will contribute to an elucidation of the dynamic aspects of PCB bioremediation in terms of roles played by PCB degraders and plant terpenes as natural inducer substrates that are ubiquitous and environmentally compatible.
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KEYWORD
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